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gfp trap a beads  (Proteintech)


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    Structured Review

    Proteintech gfp trap a beads
    Gfp Trap A Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 693 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+trap/TRAPA%2FSSR1+Antibody/pm42896554-995-13-15
    Average 96 stars, based on 693 article reviews
    gfp trap a beads - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Inherited deficiency of DIAPH1 identifies a DNA double strand break repair pathway regulated by γ-actin
    Article Snippet: Cells were incubated in lysis buffer (150 mM NaCl, 50 mM Tris HCl pH 7.5, 2 mM MgCl 2 , 1% NP40, 90 U/ml Benzonase (Novagen) and EDTA-free protease inhibitor cocktail [Roche]) for 30 min with rotation at 4 °C. .. Cell lysates were then pre-cleared at 44,000 × g at 4 °C for 30 min. For GFP-Trap, 3–5 mg of lysate was incubated with GFP-Trap agarose beads (ChromoTek) for 5 h at 4 °C. .. The resulting GFP-Trap complexes were washed with wash buffer (150 mM NaCl, 50 mM Tris HCl pH 7.5, 0.5% NP40, and complete protease inhibitor cocktail [Roche]) and analysed by SDS–PAGE.

    Article Title: SnRK2.4 and SnRK2.10 redundantly control developmental leaf senescence by sustaining ABA production and signaling
    Article Snippet: .. In brief, for immunoprecipitation of SnRK2.4-YFP, SnRK2.10-YFP, or SnRK2.2/2.3/2.6 kinases, 1100 μg or 400 μg of crude protein extracted from 8 th -10 th rosette leaves of plants 35 DAS or 45 DAS, respectively, was incubated with 50 μL of GFP-Trap®_A (Chromotek) resin or 2.5 μL anti-SnRK2.2/2.3/2.6 antibodies (Agrisera) bound to 25 μL of Protein A-Agarose (Santa Cruz Biotechnology) for 2.5 h, at 4°C with gentle rocking. .. After intensive washing, agarose beads with bound immunocomplexes were suspended in 18 μL of 20 mM Tris–HCl, pH 7.5, 150 mM NaCl supplemented with 4 μg of Myelin Basic Protein (Sigma-Aldrich) per sample.

    Article Title: Lysosomal control of proteostasis and reproductive capacity by conserved LMD-3 protein in C. elegans
    Article Snippet: .. The precleared supernatant was then incubated for 1 hour at 4°C on a rotating shaker with either GFP-Trap (ChromoTek, rtma-10) or FLAG (Thermo Fisher Scientific, A36797) magnetic agarose beads. .. Beads were washed three times with 1× phosphate-buffered saline (PBS) buffer, and proteins were eluted by resuspending in 1× sample buffer and heating at 95°C for 10 min. Eluted protein lysates were then analyzed by Western blotting using anti-mCherry rabbit (Abcam, ab213511) and anti-GFP rabbit pAb (Abclonal, AE011) antibodies.

    Article Title: Inherited deficiency of DIAPH1 identifies a DNA double strand break repair pathway regulated by γ-actin.
    Article Snippet: .. For GFP-Trap, 3–5mg of lysate was incubated with GFPTrap agarose beads (ChromoTek) for 5 h at 4 °C. .. The resulting GFPTrap complexes were washed with wash buffer (150mM NaCl, 50mM Tris HCl pH 7.5, 0.5% NP40, and complete protease inhibitor cocktail [Roche]) and analysed by SDS–PAGE.

    Article Title: Cucumber green mottle mosaic virus encodes additional small proteins with specific subcellular localizations and virulence function.
    Article Snippet: 1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China 2State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310030, China 3School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, China 4Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China 5State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China 6Department of Plant Biochemistry, Centre for Plant Molecular Biology (ZMBP), Eberhard Karls University, Tübingen D-72076, Germany 7London Research and Development Centre, Agriculture and Agri-Food Canada, London N5V 4T3, Canada

    Immunoprecipitation:

    Article Title: SnRK2.4 and SnRK2.10 redundantly control developmental leaf senescence by sustaining ABA production and signaling
    Article Snippet: .. In brief, for immunoprecipitation of SnRK2.4-YFP, SnRK2.10-YFP, or SnRK2.2/2.3/2.6 kinases, 1100 μg or 400 μg of crude protein extracted from 8 th -10 th rosette leaves of plants 35 DAS or 45 DAS, respectively, was incubated with 50 μL of GFP-Trap®_A (Chromotek) resin or 2.5 μL anti-SnRK2.2/2.3/2.6 antibodies (Agrisera) bound to 25 μL of Protein A-Agarose (Santa Cruz Biotechnology) for 2.5 h, at 4°C with gentle rocking. .. After intensive washing, agarose beads with bound immunocomplexes were suspended in 18 μL of 20 mM Tris–HCl, pH 7.5, 150 mM NaCl supplemented with 4 μg of Myelin Basic Protein (Sigma-Aldrich) per sample.

    Article Title: The Signal Peptidase MoSpc2 Orchestrates Fungal Development, Protein Secretion and Pathogenicity in Magnaporthe oryzae.
    Article Snippet: .. Purification To determine the interacting proteins of MoSpc2, MoSpc2-GFP was immunoprecipitated from hyphal protein extracts from the ΔMospc2 expressing MoSpc2-GFP fusion construct using a GFP-Trap®_A kit (ChromoTek, Germany). ..

    Gentle:

    Article Title: SnRK2.4 and SnRK2.10 redundantly control developmental leaf senescence by sustaining ABA production and signaling
    Article Snippet: .. In brief, for immunoprecipitation of SnRK2.4-YFP, SnRK2.10-YFP, or SnRK2.2/2.3/2.6 kinases, 1100 μg or 400 μg of crude protein extracted from 8 th -10 th rosette leaves of plants 35 DAS or 45 DAS, respectively, was incubated with 50 μL of GFP-Trap®_A (Chromotek) resin or 2.5 μL anti-SnRK2.2/2.3/2.6 antibodies (Agrisera) bound to 25 μL of Protein A-Agarose (Santa Cruz Biotechnology) for 2.5 h, at 4°C with gentle rocking. .. After intensive washing, agarose beads with bound immunocomplexes were suspended in 18 μL of 20 mM Tris–HCl, pH 7.5, 150 mM NaCl supplemented with 4 μg of Myelin Basic Protein (Sigma-Aldrich) per sample.

    Article Title: Cucumber green mottle mosaic virus encodes additional small proteins with specific subcellular localizations and virulence function.
    Article Snippet: 1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China 2State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310030, China 3School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, China 4Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China 5State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China 6Department of Plant Biochemistry, Centre for Plant Molecular Biology (ZMBP), Eberhard Karls University, Tübingen D-72076, Germany 7London Research and Development Centre, Agriculture and Agri-Food Canada, London N5V 4T3, Canada

    other:

    Article Title: The tumor suppressor RASSF8: A WAVE interaction partner controlling migration and cohesion of invasive border cells in Drosophila
    Article Snippet: Cells were lysed 72 h after transfection using FuGene HD (Promega).

    Purification:

    Article Title: The Signal Peptidase MoSpc2 Orchestrates Fungal Development, Protein Secretion and Pathogenicity in Magnaporthe oryzae.
    Article Snippet: .. Purification To determine the interacting proteins of MoSpc2, MoSpc2-GFP was immunoprecipitated from hyphal protein extracts from the ΔMospc2 expressing MoSpc2-GFP fusion construct using a GFP-Trap®_A kit (ChromoTek, Germany). ..

    Expressing:

    Article Title: The Signal Peptidase MoSpc2 Orchestrates Fungal Development, Protein Secretion and Pathogenicity in Magnaporthe oryzae.
    Article Snippet: .. Purification To determine the interacting proteins of MoSpc2, MoSpc2-GFP was immunoprecipitated from hyphal protein extracts from the ΔMospc2 expressing MoSpc2-GFP fusion construct using a GFP-Trap®_A kit (ChromoTek, Germany). ..

    Construct:

    Article Title: The Signal Peptidase MoSpc2 Orchestrates Fungal Development, Protein Secretion and Pathogenicity in Magnaporthe oryzae.
    Article Snippet: .. Purification To determine the interacting proteins of MoSpc2, MoSpc2-GFP was immunoprecipitated from hyphal protein extracts from the ΔMospc2 expressing MoSpc2-GFP fusion construct using a GFP-Trap®_A kit (ChromoTek, Germany). ..



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